Skip to main content
. 2020 Jan 23;11:438. doi: 10.1038/s41467-020-14332-x

Fig. 3. Cholesterol restores IFNγ production in iNKT cells lacking lipid synthesis.

Fig. 3

a Influences of indicated fatty acids on IFN-γ production in iNKT cells activated by plate-coated mCD1d-PBS57 tetramer in the presence of Tofa. b Influences of water-soluble cholesterol on IFN-γ and IL-4 production from iNKT cells activated by plate-coated mCD1d-PBS57 tetramer in the presence of Tofa. c Influences of water-soluble cholesterol on IFN-γ and IL-4 production in iNKT cells activated by plate-coated mCD1d-PBS57 tetramer in the presence of T007. d Total cholesterol or plasma membrane cholesterol in iNKT cells activated by anti-CD3 plus anti-CD28 for 24 h with or without T007, or Tofa. e Influences of Sim on IFN-γ and IL-4 production in iNKT cells activated by mCD1d-PBS57 tetramer. f Influences of water-soluble cholesterol on IFN-γ and IL-4 production in iNKT cells activated by mCD1d-PBS57 tetramer. g Influences of Tofa, C75, and cholesterol on IFN-γ and IL-4 production from iNKT cells activated by PMA plus ionomycin. h–j Distribution (h) and fluorescence intensity (i) of surface TCR, and area of immunological synapse (j) in iNKT cells activated by coverslip-coated mCD1d-PBS57 tetramer for 30 min in the presence or absence of T007, Tofa, Sim, or water-soluble cholesterol (n = 100 cells per group). Arbitary unit, A.U. Bar, 5 μm. k P-LCK (Y394), LCK, P-LAT (Y191), LAT in iNKT cells activated by plate-coated anti-CD3 plus anti-CD28 for 20 min in the presence or absence of T007, Tofa, Sim, or water-soluble cholesterol. Data are means ± SEM of three independent experiments (a, d, g) or nine biological replicates (b, c, e, f), pooled from three independent experiments, or are representative of three independent experiments (h, k). Data were analyzed by Mann–Whitney test (a, d, g) or unpaired Student’s t-test (b, c, e, f, i, j). *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.