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. 2020 Jan 23;11:437. doi: 10.1038/s41467-019-14218-7

Fig. 2. SK1 downregulation increases the CD8 + /Treg ratio and reduces tumor growth.

Fig. 2

a SK1 enzymatic activity was measured in Yumm cells transfected with a control shRNA (shCtrl, black column) or two SK1-targeted shRNA (white and gray columns) (means ± SEM of 5 independent experiments). b Cell proliferation (n = 4). Results represent means of 3 independent experiments. c shCtrl (black points), shSK1(1) or shSK1(2) (white points) Yumm cells were injected in wild-type (WT) C57BL/6 mice (n = 12 mice/group). Arrows indicate tumor regression of shSK1 tumors. d shCtrl or shSK1(1) Yumm cells were injected in NOD scid gamma (NSG) immunodeficient mice (n = 5 mice/group). Tumor volume was determined at the indicated days. Tumor volumes, presented as means ± SEM, are representative of at least two independent experiments. e, f shCtrl or shSK1(1) Yumm cells were injected in C57BL/6 mice, and TIL content was analyzed by flow cytometry on day 11. Percentages of CD8 + (upper panel) and CD4 + Foxp3 + (Treg; lower panel) T cells and CD8 + /Treg ratio were calculated. e Representative stainings; values indicate the percentage of cells in the quadrant. f Each symbol represents an independent tumor (n = 9 mice/group). Results are representative of at least two independent experiments. Data were compared using Kruskal–Wallis test with Dunn’s correction (a, b), two-way ANOVA test (c, d) or Mann–Whitney test (e and f).