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. 2020 Jan 23;10:1058. doi: 10.1038/s41598-020-58032-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pale martin pachytene spermatocytes with one (a,d), two (b,e), and three (c,f) copies of GRC. (a–c) Cells after immunostaining with antibodies against SYCP3 (red), centromere proteins (blue) (a,b) and MLH1 (green) (a,b). (d–f) The same cells after FISH with the pale martin GRC paint probe. Arrowheads point to GRCs. Inserts show zooms at the GRC with enhanced brightness and contrast (a–c) and schematic representations of GRC SCs (d–f). Note MLH1 foci at both ends of the partially paired GRC bivalent (b). Bar – 5 µm.