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. 2020 Jan 23;10:1058. doi: 10.1038/s41598-020-58032-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization of GRCs on the spreads of pale martin germ cells after FISH with the pale martin GRC probe (green), and DAPI staining (blue). (a–c) Spermatogonia with one (a) and two (b,c) GRC copies. (d) Pachytene spermatocyte immunolabelled with antibodies against SYCP3 (red) with one GRC copy. Insert shows zoom at the GRC with enhanced brightness and contrast. (e) Post-meiotic cells and ejected GRC as round chromatin body. Arrowheads point to GRCs. Bar – 10 µm.