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. 2020 Jan 23;10:1023. doi: 10.1038/s41598-020-57668-6

Table 4.

Effect of dietary soaking of cereals with or without carbohydrase supplementation on the coefficient of apparent ileal digestibility (AiD, %), apparent total tract digestibility (ATTD, %), digestible energy (MJ/kg on a DM basis), pH and volatile fatty acid (VFA, mmol/kg) concentrations in the ileal and caecal digesta of grow-finisher pigsa.

Cereal soaking (Csoak)b: Fresh Fresh Soak Soak SEMd P-Value
Enzyme (ENZ)c: + + ENZ Csoak ENZ*Csoak
AiD
Dry matter 62.8 64.2 63.0 63.8 1.70 0.51 0.95 0.87
Organic matter 64.2 66.6 64.3 66.1 1.69 0.22 0.90 0.87
Crude protein 56.5 61.6 59.5 61.1 2.16 0.10 0.59 0.43
Gross energy 60.5 63.0 61.8 62.5 1.81 0.39 0.81 0.62
ATTD
Dry matter 81.7 85.1 83.3 85.1 0.48 <0.001 0.11 0.12
Organic matter 83.0b 86.8a 84.6c 86.7a 0.42 <0.001 0.10 <0.05
Crude protein 76.9 84.2 78.8 83.4 0.82 <0.001 0.51 0.13
Gross energy 79.6c 83.9a 81.8b 84.0a 0.55 <0.001 <0.05 <0.05
Digestible energy 14.8c 15.4a 15.1b 15.4a 0.10 <0.001 <0.001 <0.001
Ileal digesta
pH 6.5 6.3 6.3 6.4 0.23 0.74 0.72 0.44
Total VFAe 50.7 44.6 47.6 44.3 10.80 0.65 0.86 0.89
Caecal digesta
pH 5.7 5.7 5.6 6.0 0.16 0.13 0.42 0.22
Acetate 92.1 90.0 106.7 86.7 5.44 <0.05 0.29 0.11
Propionate 25.1 21.3 28.5 19.1 2.26 <0.01 0.78 0.20
Isobutyrate 1.4 1.2 1.2 1.1 0.17 0.29 0.47 0.72
Butyrate 19.0 13.8 20.9 13.0 1.68 <0.01 0.78 0.44
Isovalerate 1.0 1.2 1.0 1.2 0.18 0.80 0.27 0.96
Valerate 2.8 2.0 2.7 1.7 0.34 <0.05 0.65 0.75
Total VFA 141.4 129.7 161.5 122.7 9.09 <0.05 0.46 0.14
Acetate:Propionate 3.7 4.3 0.8 6.0 0.83 0.12 0.32 0.37
Protein-derived VFA 5.2 4.1 5.2 4.0 0.45 <0.05 0.86 0.91

aValues within a row with a different superscript are statistically different (P < 0.05).

bSoaking the cereal fraction of the diet prior to feeding (fresh = 0 h soaking, soak = 3 h soaking).

cEnzyme supplementation with a Xylanase and β-glucanase complex [unsupplemented (−), supplemented (+)].

dSEM = standard error of the mean.

eConcentrations of each individual VFA were not significantly different between treatments, and therefore only the total VFA concentration is shown.