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. 2020 Jan 23;10:1073. doi: 10.1038/s41598-020-58062-y

Figure 1.

Figure 1

NleB glycosylates GshB R256. (A) Heatmap of Z-scored ion intensities of GshB peptides demonstrates that glycosylated R256 is observed only within WT GshB samples in both in vivo and in vitro glycosylation assays. The abundance of non-glycosylated peptides 109GTLIVNKPQSLRDCNEK125, 88DPPFDTEFIYATYILERAEEK108 and 145AQLKAFWEK153 are unaltered across assays (B) EThcD spectra of the in vivo glycosylated GshB peptide 254IARQIGPTLK263 confirms glycosylation is localized to R256. (C) Western blot analysis of in vitro GshB glycosylation assays. (D) Western blot analysis of in vivo GshB glycosylation assays; Sup, culture supernatant; Pel, bacterial lysate.