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. 2020 Jan 23;10:1073. doi: 10.1038/s41598-020-58062-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro GSH assays. (A) Coomassie blue staining of purified proteins used in GSH assays. (B) Western blot analysis of the GshB glycosylation state from GSH assays. (C) GSH production as a function of time from reactions containing either WT or GshB(256A) purified after their co-expression with either WT or NleB(AAA).