Table 1.
Plasmids and strains used in this study.
| Plasmid | Source |
|---|---|
| FLAG-NleB (C. rodentium) | 1 |
| FLAG-NleB (C. rodentium) (DAD221–223/AAA) | 1 |
| GST-NleB (C. rodentium) | 17 |
| GST-NleB (C. rodentium) (DAD221–223/AAA) | 17 |
| GST-NleB1 (EHEC) | 17 |
| GST-NleB1 (EPEC) | 17 |
| GST-NleB2 | 17 |
| GST-SseK1 (S. enterica) | 17 |
| GST-SseK2 (S. enterica) | 17 |
| GST-SseK3 (S. enterica) | 17 |
| His-FADD | 17 |
| His-GshA | This study |
| His-GshB | This study |
| His-GshB(R256A) | This study |
| FLAG-His-GshB | This study |
| FLAG-His-GshB(R256A) | This study |
| His-CesT | This study |
| His-GST | Novagen |
| Strain | Source |
| C. rodentium DBS100 | 44 |
| C. rodentium DBS100 ΔnleB | 1 |
| C. rodentium DBS100 ΔnleB/pFLAG-CTC-nleB | 1 |
| C. rodentium DBS100 ΔnleB/pFLAG-CTC-nleB DAD221–223/AAA | 1 |
| C. rodentium DBS100 ΔcesT | 45 |
| C. rodentium ΔgshB | This study |
| C. rodentium ΔgshB/pFLAG-CTC gshB | This study |
| C. rodentium ΔgshB/pFLAG-CTC gshB(R256A) | This study |
| S. enterica | 46 |
| S. enterica ΔsseK1 | 46 |
| S. enterica ΔsseK2 | 46 |
| S. enterica ΔsseK3 | 46 |
| S. enterica ΔsseK1ΔsseK2 | 46 |
| S. enterica ΔsseK1ΔsseK3 | 46 |
| S. enterica ΔsseK2ΔsseK3 | 46 |
| S. enterica ΔsseK1ΔsseK2ΔsseK3 | 46 |
| E. coli BL21(DE3) x pET28a-gshA | This study |
| E. coli BL21(DE3) x pET28a-gshB | This study |
| E. coli BL21(DE3) x pET28a-gshB(R256A) | This study |
| E. coli BL21(DE3) x pET42a-nleB | This study |
| E. coli BL21(DE3) x pET42a-nleB DAD221-223/AAA | This study |
| E. coli BL21(DE3) x pET42a-nleB1 (EHEC) | 17 |
| E. coli BL21(DE3) x pET42a-nleB1 (EPEC) | 17 |
| E. coli BL21(DE3) x pET42a-nleB2 | 17 |
| E. coli BL21(DE3) x pET42a-sseK1 | 17 |
| E. coli BL21(DE3) x pET42a-sseK2 | 17 |
| E. coli BL21(DE3) x pET42a-sseK3 | 17 |
| E. coli O111:NM R82F2 | 36 |