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. 2020 Jan 23;11(1):58. doi: 10.1038/s41419-020-2231-8

Fig. 5. TCTP and p-AKT proteins were significantly increased in the in vitro liver regeneration (LR) model.

Fig. 5

a The RT-PCR analysis for the mRNA expression of PCNA and Ki67 in primary hepatocytes and the AML12 cell line treated with CM or LPS-CM, in order to testify the efficiency of the LPS-CM model. GAPDH was used as a reference gene. The LPS-CM (or CM) was obtained from LPS-treated (or PBS-treated) RAW 264.7 macrophages (20 μg/mL for 2 h), and the hepatocytes were harvested after incubation with LPS-CM (or CM) for 2 h. b Representative western blot analysis for the protein expression of TCTP, AKT and p-AKTSer473 in the LPS-CM (or CM) treated hepatocytes. c The average gray value of each blot was quantified using the Image LabTM software. All experiments were conducted in triplicate. The results were presented as mean ± standard deviation (SD); **P < 0.01; ***P < 0.001 vs. control (two-tailed Student’s t test).