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The sequences of primers used in RQ‐MSP and BSP
| Forward (5’→3’) | Reverse (5’→3’) | Product (bp) | |
|---|---|---|---|
| RQ‐MSP | |||
| M | GGATGAGTTTTGAGTCGTTT | CCATACAAACCGCTCACTCC | 129 |
| U | TAGGATGAGTTTTGAGTTGT | ATAATCCCATACAAACCACT | 137 |
| BSP | AGGATTTTTTGGTGATTTTTG | TCACCCTCTAACTACATAATCCC | 200 |
Abbreviations: BSP, bisulfite‐sequencing PCR; RQ‐MSP, real‐time quantitative methylation‐specific PCR.
