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. 2020 Jan 23;11:445. doi: 10.1038/s41467-020-14283-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic of Porcupine inhibition with Porcupine-i during bile duct regeneration induced by DDC or TAA. b mRNA expression of Wnt-PCP pathway target genes Ctgf and Mmp7 in isolated bile ducts following Porcupine-i treatment, normalised to the housekeeping gene Ppia. c Immunofluorescent staining of biliary epithelial cells (Keratin-19-positive, red) with phosphorylated JNK^T183/Y185 (upper panels) or phosphorylated c-JUN^S73 (lower panels), green. White arrows denote positivity in biliary epithelial cells, yellow arrows show positivity in other, non-keratin-19-positive cells. Positivity of phosphorylated JNK^T183/Y185 and phosphorylated c-JUN^S73 quantified in dot plots and represented as a proportion of total biliary epithelial cells counted. d Histological quantification of Keratin-19 (biliary epithelial cells), Desmin (fibroblasts), Picrosirius Red, PSR (fibrilar collagens) and Collagen-1 in livers of mice treated with either DDC or TAA, following Porcupine inhibition. Scale bar = 50 µm. p.v.—portal vein, b.d.—bile duct. Source data are provided as a Source Data file. In all comparisons, a Student’s t test is used. Box–whisker plots represent min–max range of the data. In dot plots, data are presented as mean ± S.E.M. Each data point (N) represents an individual animal.