Fig. 5. Inhibition of Wnt-PCP reduces the formation of biliary scars.

a Schematic representing the treatment strategy of Vangl2^S464N mutant mice treated with either DDC or TAA to initiate bile duct damage and regeneration. b Relative mRNA expression of Wnt-PCP target genes Ctgf and Mmp7 in DDC and c TAA-induced biliary regeneration normalised to Ppia. d Image quantification of Picrosirius Red, PSR (fibrillar collagen) and Collagen-1 in DDC or TAA-induced biliary injury. e Quantification of the number of BECs (through Keratin-19 immunohistochemistry) following DDC (left panel) or TAA (right panel) in Vangl2^S464N mutant mice compared with controls. f Desmin positivity (fibroblasts) in mice with DDC or TAA-induced biliary regeneration. g Image analysis and quantification of CTGF protein levels in mice with DDC (upper panel) or TAA- (bottom panel) induced biliary injury. Source data are provided as a Source Data file. In graphs where two groups are included, a Student’s t test is used. When comparing multiple groups, a two-way ANOVA with post hoc correction for multiple testing is used. Box–whisker plots represent min–max range of the data. In dot plots, data are presented as mean ± S.E.M. Each data point (N) represents an individual animal.