Fig. 2. Biphasic pole growth: NETO and cell division are independent events.
a Elongation of a representative newborn pole through three generations measured by time-lapse AFM. Gray: AFM data; orange/blue: bilinear fit. See Supplementary Fig. 4. b Schematic of biphasic growth of a newborn pole with a rate change (NETO). c Schematic of biphasic NETO growth of a cell over one cell cycle. d Left: elongation speeds of newborn poles during the pre-NETO phase (slow or no growth) and the post-NETO phase (fast growth). Right: pre-NETO time and interdivision time. Data from time-lapse AFM images (20 poles). Bars indicate averages and standard deviations. e Typical elongation curve of a newborn cell pole (left) and atypical curves with early NETO before division (center) and late NETO after division (right). Each curve corresponds to one of the data points in d. Gray: AFM data; orange/blue: bilinear fit.