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. 2020 Jan 23;11:452. doi: 10.1038/s41467-019-14088-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Elongation dynamics of representative old and new cell poles of M. smegmatis and M. tuberculosis between birth and division. Points: phase-contrast microscopy data. Lines: linear fit (blue) or bilinear fit (orange). b Pre-NETO phase vs. interdivision time in M. smegmatis (38 poles), M. tuberculosis (14 poles), M. abscessus (6 poles), and M. marinum (6 poles). Each symbol represents a new cell pole. Black lines indicate the expected pre-NETO time and interdivision time corresponding to unipolar growth or bipolar growth. c Asymmetry of old pole and new pole growth between birth and division in individual cells of M. smegmatis, M. abscessus, M. marinum, and M. tuberculosis, expressed as the ratio (pre-NETO time)/(interdivision time). Bars indicate averages and standard deviations. d Calculated spatial pattern of cell wall age for individual cells within a microcolony of M. smegmatis based on time-lapse phase-contrast microscopy. e Calculation of the partitioning of cell wall material inherited by the daughter cells from the original mother cell in M. smegmatis (top) and M. tuberculosis (bottom), based on the average values calculated from the experimental data in c and assuming symmetric division for simplicity.