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. 2020 Jan 23;10:1038. doi: 10.1038/s41598-020-57874-2

Figure 2.

Figure 2

Activation or overexpression of BK increases macrophage phagocytosis of large particles. (A–D) BK agonist NS1619 significantly increased the ingestion of large beads and SRBCs but not small beads in RAW264.7 cells. RAW264.7 cells were treated with 20 μM NS1619 for 30 min and then incubated with opsonized 4.5 μm or 0.8 μm polystyrene beads or SRBCs at 37 °C for 60 min. Scale bars: 10 μm. (E–H) NS1619 significantly increased the ingestion of large beads (4.5 μm) and SRBCs in BMMs from wild-type but not BK KO mice. ML-SA1 rescued the defective phagocytosis of large particles in BMMs derived from BK KO mice. BMMs were pre-treated with 20 μM NS1619 or 10 μM ML-SA1 for 30 min and then incubated with large beads or SRBCs at 37 °C for 60 min. Scale bars: 10 μm. (I–K) Overexpression of BK in RAW264.7 cells increased the ingestion of large beads and SRBCs. For particle ingestion, experiments were repeated three times with triplicated samples each time for all conditions. Totally, 150–200 cells were counted for each condition.