Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 23;10:1038. doi: 10.1038/s41598-020-57874-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Large particle binding causes the recruitment of lysosomal membrane proteins to the phagocytic sites in RAW264.7 macrophages. (A–D) Selected frames from time-lapse confocal microscopy of RAW 264.7 macrophages transfected with indicated plasmids that were exposed to 4.5 µm IgG-coated polystyrene beads. In response to 4.5 μm IgG-coated particles, BK channels and other lysosomal membrane proteins were simultaneously recruited to the forming phagosomes within 4 min after phagocytosis initiation. The center of the particle is indicated with an asterisk. (E) Comparable recruitment kinetics of BK, TRPML1, Syt VII and Lamp1 to the particle-containing phagosomes in RAW264.7 macrophages were detected. Paxilline (1 µM) treatment inhibited the recruitment of lysosomal membrane proteins.