Fig. 6. NPY-dependent signaling mediates the increase in feeding as well as the induction of insulin resistance upon chemogenetic stimulation of AgRP neurons.

a Expression of hM3DGq occurs exclusively in AgRP neurons of the ARC. DAPI is depicted in gray in the DMH image (n = 5 mice). Scale bar = 100 µm; applies to all images; scale bar = 20 µm; applies to insets. b Food intake upon hM3DGq-induced stimulation of AgRP neurons in the presence and in the absence of NPY (n = 10 mice for NPY^wt/wt, hM3DGq^AgRP; NPY^wt/wt and NPY^Δ/Δ, and n = 11 mice for hM3DGq^AgRP; NPY^Δ/Δ). c NPY is necessary for the acute food consumption upon AgRP neuron stimulation (n = 10 mice for NPY^wt/wt, hM3DGq^AgRP; NPY^wt/wt and NPY^Δ/Δ, and n = 11 mice for hM3DGq^AgRP; NPY^Δ/Δ). All animals were injected with CNO. Data are shown as mean ± s.e.m. Statistical analysis is represented by *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ^####p ≤ 0.0001 as determined by two-way ANOVA followed by Tukey post hoc test, except for panel c where a Sidak post hoc test was performed. # represents comparisons between NPY-expressing and NPY-deficient animals. Source data are provided as a Source Data file.