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. 2020 Jan 23;10:1032. doi: 10.1038/s41598-020-57559-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological analysis of wound tissue from day 14 using hematoxylin and eosin (H&E) (magnification 10× , and 40×). (A,D and G) compared the epidermis and the extracellular matrix which is thinner in (A) (untreated control wounds) compared to (D and G) (freeze or CaCl₂-PdB treated wound). (B,E and H) 40× magnification of the Histologic wound shows the separated epidermis in B (untreated control wounds) and new angiogenesis in (E and H) (freeze or CaCl₂-PdB treated wound). (C,F and I) 40× magnification of the Histologic wound shows the inflammation and cell aggregation in (C) (untreated control wounds) and collagen production (F and I) (freeze or CaCl₂-PdB treated wound).