Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation

. 2019 Dec 25;36(4):241–251. doi: 10.5511/plantbiotechnology.19.1025a

Name	MYB type	Number of amino acid residues	GmCHS8	GmIFS1	GmIFS2
GmMYB4	R2R3	406	++	++	+
GmMYB77	R2R3	322	++	++	++
GmMYB102	R2R3	275	++	++	++
GmMYB128	R2R3	287	+	+/−	+/−
GmMYB202	R2R3	377	–	–	–
GmMYB250	R2R3	330	+	++	+
GmMYB280	R2R3	252	+	++	+/−
GmMYB399	R2R3	452	+/−	+/−	+/−
GmMYB415	R2R3	289	+/−	+/−	+/−
GmMYB467	R2R3	320	+	+/−	-
GmMYB502	R2R3	284	++	++	+
GmMYB522	R2R3	373	++	+/−	+/−
GmMYB176	R1	285	++	++	+

MYB types are assigned according to Du et al. (2012), Aoyagi et al. (2014) and soybean genome information from http://phytozome.jgi.doe.gov/. ++, ≥2-fold activation; +, ≥1.5-fold activation;–, ≥2-fold suppression and -, ≥1.5-fold suppression of GUS activity compared to the reporter construct only. +/−, no change in GUS activity.