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. 2019 Dec 25;36(4):233–240. doi: 10.5511/plantbiotechnology.19.1016a

Figure 2. Agrobacterium-mediated transformation and GFP expression in transgenic soybean. (A) Wounding of half-seed explant by using a micro-brush; (B) Inoculation with Agrobacterium in liquid co-cultivation media (CM); (C) Co-cultivation of half-seed explants with Agrobacterium on solid CM medium lined with filter paper; (D) Four weeks after selection on shoot induction medium containing 6 mg l−1 bialaphos; (E, F) Stable GFP expression during the shoot induction selection stage (F under fluorescent); (G) Rooting of putative transgenic plant; (H) Wild type (left) and T0 transgenic plant (right) in greenhouse; (I, J) Stable GFP expression of silique pod (J under fluorescent); (K, L) GFP expression on heterozygous T2 seeds (L under fluorescent); (M) homozygous T2 transgenic soybean seeds under fluorescent; (N, O, P, Q, R, S) Stable GFP expression of T3 transgenic plant (O, Q, S under fluorescent).

Figure 2. Agrobacterium-mediated transformation and GFP expression in transgenic soybean. (A) Wounding of half-seed explant by using a micro-brush; (B) Inoculation with Agrobacterium in liquid co-cultivation media (CM); (C) Co-cultivation of half-seed explants with Agrobacterium on solid CM medium lined with filter paper; (D) Four weeks after selection on shoot induction medium containing 6 mg l−1 bialaphos; (E, F) Stable GFP expression during the shoot induction selection stage (F under fluorescent); (G) Rooting of putative transgenic plant; (H) Wild type (left) and T0 transgenic plant (right) in greenhouse; (I, J) Stable GFP expression of silique pod (J under fluorescent); (K, L) GFP expression on heterozygous T2 seeds (L under fluorescent); (M) homozygous T2 transgenic soybean seeds under fluorescent; (N, O, P, Q, R, S) Stable GFP expression of T3 transgenic plant (O, Q, S under fluorescent).