Table 2.
Quality control of the Rhinella arenarum transcriptome. Quality scores were calculated using TRANSRATE v1.0.3, BUSCO v3.0.2, and DETONATE v1.9 before and after the CD-HIT clustering tool.
| Before CD-HIT | After CD-HIT | |
|---|---|---|
| TRANSRATE v1.0.3 | ||
| Transrate Assembly Score | 0.0457 | 0.158 |
| Transrate Optimal Score | 0.1172 | 0.2092 |
| Transrate Optimal Cutoff | 0.129 | 0.0928 |
| good contigs | 163674 | 173616 |
| p good contigs | 0.66 | 0.87 |
| BUSCO v3.0.2 | ||
| Complete BUSCOs (C) | 973 (99.4%) | 972 (99.4%) |
| Complete and single-copy BUSCOs (S) | 507 (51.8%) | 740 (75.7%) |
| Complete and duplicated BUSCOs (D) | 466 (47.6%) | 232 (23.7%) |
| Fragmented BUSCOs (F) | 0 (0.0%) | 0 (0.0%) |
| Missing BUSCOs (M) | 5 (0.6%) | 6 (0.6%) |
| Total BUSCO groups searched | 978 | 978 |
| DETONATE v1.9 | ||
| Score | −14949658702 | −15357930779 |
| BIC_penalty | −2381204 | −1893606,56 |
| Prior_score_on_contig_lengths_(f_function_canceled) | −809045 | −628519,94 |
| Prior_score_on_contig_sequences | −339449528 | −220853915,1 |
| Data_likelihood_in_log_space_without_correction | −14607924788 | −15135245313 |
| Correction_term_(f_function_canceled) | −905861 | −690575,74 |