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. 2020 Jan 23;11(1):51. doi: 10.1038/s41419-020-2253-2

Fig. 3. Mitochondria of KIKO mice show altered mitochondrial morphology and respiratory function.

Fig. 3

a Representative transmission electron microscopy pictures of BAT from 6-month-old WT and KIKO mice are reported in left panels (×5800). Images with high power field (×18,500) are reported in right panels. A representative mitochondrion of KIKO mice with altered ultrastructure was evidenced with an asterisks (*). LD lipid droplets; N nucleus; M mitochondria. b Oxygen consumption determined through a polarographic method on crude mitochondria isolated from BAT of 6 months old mice (n = 6 each group; ****p < 0.0001 vs. WT). c Representative BAT histology images after staining with H&E (n = 3 each group). Scale bars, 25 µm. d, e Representative immunoblots d of total HSL, phospho-active (p-HSL660) and phospho-inactive HSL (p-HSL565), PKA phosphorylated substrates (PKA p-substrates), UCP1 and NRF2 in total BAT homogenates, and densitometric analyses of the immunoreactive bands e. HSL was used as loading control (n = 6 each group, *p < 0.05, **p < 0.01 vs. WT). f Western blot analysis of NRF2 in nuclear and cytoplasmic fraction of BAT (n = 2 each group). Lamin A/C and GAPDH were used as loading control for nuclear and cytoplasmic fraction, respectively. g RT-qPCR analysis of Fxn, Nrf2, and its down-stream genes in BAT (n = 6; *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT).