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. 2020 Jan;59(1):30–36. doi: 10.30802/AALAS-JAALAS-19-000027

Figure 1.

Figure 1.

Schematic timeline of the experimental period. Phase 1: burrowing behavior test (BUR) used to assess the effect of grid floor housing (BURgrid); Phase 2: burrowing used to assess the effect of repeated exposure to isoflurane anesthesia (BURiso1 and BURiso2) and repeated intraperitoneal injections (BURip); Phase 3: nest-building activity (NBA) used to assess the effect of repeated exposure to isoflurane anesthesia (NBAiso3 and NBAiso4). A1–22, assessment of body weight and fur status; bBUR, baseline burrowing test; BURgrid, burrowing test after exposure to grid-floor housing; BURip, burrowing after repeated intraperitoneal injections; BURiso1, burrowing after the 1st exposure to isoflurane; BURiso2, burrowing after the 2nd exposure to isoflurane; bNBA, baseline nest building activity test; collection of feces for fecal corticosterone metabolites; GRID, grid-floor housing; intraperitoneal injections; Iso, isoflurane; NBAiso3, nest-building activity after the 3th exposure to isoflurane; NBAiso4, nest-building activity after the 4th exposure to isoflurane; sucrose preference test.