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. 2020 Jan 17;11:382. doi: 10.3389/fnagi.2019.00382

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Copyright © 2020 Verma and Ravindranath.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ca_V1.3₄₂ and Ca_V1.3_42A mRNA expression levels in mouse brain regions as measured by qRT-PCR. (A) Expression of Ca_V1.3_42A (F_(2,12) = 15.54, p = 0.0005), (B) Ca_V1.3₄₂ (F_(2,12) = 73.22, p < 0.0001) in the ventral midbrain (MB) in comparison to expression profiles in the cortex (CT) and striatum (ST). Panels (C–E) describe the relative expression of Ca_V1.3_42A to Ca_V1.3₄₂ in ventral midbrain (p = 0.0281, t = 2.675, df = 8), cortex (p < 0.0001, t = 17.78, df = 8) and striatum (p < 0.0001, t = 10.86, df = 8), respectively. Data were normalized to mean of 18S rRNA, β-actin and GAPDH as endogenous controls. Each dot in the figure represents an individual animal. One-way ANOVA with Newman–Keuls test was performed for multiple comparisons and unpaired, two-tailed student’s t-test was performed for pair-wise comparisons on the data. Data represented as mean ± SEM. *p < 0.05.