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. 2020 Jan 17;10:3063. doi: 10.3389/fmicb.2019.03063

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Copyright © 2020 Hwang, Park, Lee and Choi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deletion of brpR and brpT eliminates cabA expression. (A,B) Total RNA was isolated from biofilms of the V. vulnificus strains grown in microtiter plates. The level of cabA transcript was determined by qRT-PCR, and the parent strain was set to 1. Error bars represent the SD from three independent experiments. ^∗∗, p < 0.005 relative to the parent strain; ns, not significant. Parent (pJK1113), parent strain; ΔbrpR (pJK1113), brpR mutant; ΔbrpT (pJK1113), brpT mutant; ΔbrpR (pJK1113::brpR) and ΔbrpT (pJK1113::brpT), complemented strains; ΔbrpRΔbrpT (pJK1113), brpR brpT double mutant; ΔbrpRΔbrpT (pJK1113::brpR) or ΔbrpRΔbrpT (pJK1113::brpT), brpR brpT double mutant expressing BrpR or BrpT, respectively.