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. 2020 Jan 17;10:3063. doi: 10.3389/fmicb.2019.03063

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Copyright © 2020 Hwang, Park, Lee and Choi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription start site and sequence of the P_cabA regulatory region. (A) The transcription start site of P_cabA was determined by primer extension of the RNA isolated from biofilms of the parent strain. Lanes G, A, T, and C represent the nucleotide sequencing ladders. The asterisk indicates the transcription start site of P_cabA. (B) The transcription start site of P_cabA is indicated by a bent arrow, and the position of the putative –10 and –35 regions are underlined. The sequences for binding of BrpS (BRPSB; gray box) and BrpT (BRPTB; white box) were determined later in this study (Figures 6C,D).