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. 2020 Jan 17;10:1580. doi: 10.3389/fphys.2019.01580

TABLE 2.

Primer sequences used for real-time PCR and the GenBank number of the PCR products.

Primer Sequences (5′ to 3′)2 GenBank No.
IFN-α1 F-GGACATGGCTCCCACACTAC R-GGCTGCTGAGGATTTTGAAGA XM-004937097.1
TNF-α1 F-AATTTGCAGGCTGTTTCTGC R-TATGAAGGTGGTGCAGATGG NM-204267
IL-61 F-AGGACGAGATGTGCAAGAAGTTC R-TTGGGCAGGTTGAGGTTGTT NM-204628.1
IGF-11 F-TGTACTGTGCTCCAATAAAGC R-CTGTTTCCTGTGTTCCCTCTACTTG NM-416323
β-actin F-GAGAAATTGTGCGTGACATCA R-CCTGAACCTCTCATTGCCA NM-205518

1Abbreviations: IFN-α, interferon-α; TNF-α, tumor necrosis factor; IL-6, interleukin-6; IGF-I, insulin-like growth factor 1. 2F, forward primer, R, reverse primer.