FIGURE 4.

Changes in viral ORF transcript levels at different stages of growth in the AfuCV41362-infected strain. (A) RNA levels were analyzed by real-time PCR during the fungal life-cycle, with time points after expose to liquid medium as follows: 0 h (conidia), 6 h (germination), and later stages of mycelial development (12 h, 24 h, and days 3 and 6). (B) RNA levels were analyzed by real-time PCR during sporulation, with time points as follows: 0 (day 6, mycelia), 6 (vesicle formation), 12 (phialide formation), and 24 h (conidial formation). Data values were normalized to that of the internal control tef-1, and presented as mean ± SD of three independent experiments.