FIGURE 6.

(A) Ca²⁺ influx and (B) superoxide anion generation in endothelial cells incubated with vehicle (white circles), mitochondrial DNA from control (cmDNA, gray circles) or diabetic (dmDNA, black circles) mice in the presence of vehicle (white triangles) or a Ca²⁺ chelator (Bapta, gray and black triangles). On top, representative images of western blot and, at the bottom, densitometric analysis of the expression of (C) caspase-1 and pro-caspase-1 in endothelial cells primed with lipopolysaccharide (LPS) and incubated with vehicle (white squares), mitochondrial DNA from control (cmDNA, gray circles) or diabetic (dmDNA, black circles) mice in the presence of vehicle (white triangles) or a Ca²⁺ chelator (Bapta, gray and black triangles). Each point/column represents the mean ±SEM. ^∗P < 0.05 vs. vehicle, ^#P < 0.05 vs. dmDNA. N = 5–12, one-way ANOVA (A–C).