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. 2020 Feb;372(2):216–223. doi: 10.1124/jpet.119.262410

Fig. 3.

Fig. 3.

Effect of LY344864 on spinal cord EB dye accumulation. Mice were subjected to moderate SCI using an 80 kdyn force-controlled impactor-induced contusion model followed by daily administration of vehicle or LY344864 (2 mg/kg i.p.) beginning 1 h postinjury. Subsets of mice were euthanized 1, 3, and 7 days post-SCI. Prior to euthanasia, mice were intraocularly injected with EB, and the dye was allowed to circulate for 2 h. One centimeter of spinal cord surrounding the injury epicenter was extracted and analyzed for dye accumulation. A subset of mice were transcardially perfused and fixed with 4% PFA and the spinal cords extracted for representative images (A). The injury site was also collected from a subset of mice at 3 days (B) and 7 days (C) post-SCI and analyzed for protein expression. Data represent five mice per group and are expressed as mean ± S.E.M. *P < 0.05 compared with Sham and #P < 0.05 compared with SCI + Vehicle by one-way ANOVA followed by Tukey’s post hoc test.