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Western blot expressions of target proteins with strophanthidin treatment. Expression of cell cycle-regulating proteins such as Chk1, Chk2, CDK6, and cyclin D1 in three cancer cell lines and statistical analysis of cell cycle-regulating proteins in strophanthidin-treated cancer cells. Blots were compared with those of GAPDH expression to compare equal loading of samples. Representative blots from three independent experiments are shown (P < 0.05). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
