Figure 5.

(A) Enzyme-linked immunosorbent assay (ELISA) for the expression analysis of caspases on strophanthidin treatment. Overexpressions of caspases 3, 7, 8, and 9 in strophanthidin-treated cells along with controls were observed in strophanthidin-induced MCF-7, A549, and HepG2 cells. (B) MCF-7, A549, and HepG2 cells were treated with lethal doses of Lanatoside C for the indicated times, and lysates were prepared. Western blot analysis was performed with antibodies to detect activation caspases 3, 7, 8, and 9. Also, the differential expression of p53 was observed. Experiments were performed in triplicates (n = 3), and the P is <0.05. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.