Fig. 3.

Phenobarbital induced p-Ser100 RORα onto the PBREM and OARE motifs in human primary hepatocytes. (A) ChIP-PCR assays were performed in human primary hepatocytes using antibodies against RORα, p-Ser100 RORα, HNF4α, and RXRα to find interaction of these factors with PBREM, 2B6-RORE, and OARE. Though both RORα and p-Ser100-RORα were detected at RORE before and after PB treatment, PB initiated increase of DNA bands corresponding to RORα and p-RORα interaction with PBREM and OARE. (B) The same set of antibodies and PCR primers was used for quantitative ChIP-PCR analysis for OARE and PBREM. The asterisk (*) between the groups represents statistically significant difference (*P < 0.05; **P < 0.01, ***P < 0.001). Mean and S.D. are shown for each transfected group (n = 6).