Fig. 6.

Phenobarbital induced change in CYP2B6 chromatin conformations in human primary hepatocytes. 3C assay was performed using a restriction enzyme AluI and forward and reverse primers starting at −1495 and −195 upstream CYP2B6 transcription site, respectively. After digestion and ligation, the primers amplified a 207-bp DNA fragment only in PB-treated samples, as confirmed by agarose gel electrophoresis and subsequent sequencing.