Skip to main content
. 2020 Mar;97(3):191–201. doi: 10.1124/mol.119.118273

TABLE 1.

Primers and oligos used in gel mobility shift (EMSA), ChIP, and 3C assays

Application/Motif Forward Sequence (5′–3′) Reverse Sequence (5′–3′)
EMSA
NR1/PBREM TCAGGG​TCAGGA​AAGT​ACAGTT AAC​TGT​ACT​TTC​CTG​ACC​CTG​A
2B6-RORE TCTTGACCTCAATTGATC GATCAATTGAGGTCAAGA
DR1/OARE ACCTGG​ACTTTGA​TATCTC GAG​ATA​TCA​AAG​TCC​AGG​T
ChIP
PBREM TTA​CTG​TGT​GTA​AAG​CAC​TTC GAC​AAA​CAG​TCC​TAT​TTG​TAA​G
2B6-RORE CCG​AGT​AGC​TGG​GAT​TAA​AAG​TAC​CCA CTT​CCC​AAC​GTG​CTG​GGA​TTA​CAG
OARE GGA​CAA​ATG​CAT​GCA​AGC​AC TGA​CCT​GAT​GCC​TAT​CCC​TTT​AC
3C
PBREM/OARE CTG​TCA​GAG​GAT​GTG​TGG​GTG GGC​AGG​GAT​ACA​GTG​GTA​GAG