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. 2020 Jan 17;10:3088. doi: 10.3389/fimmu.2019.03088

Figure 2.

Figure 2

The HLA-DRB1*01:01 recognizes encephalitogenic and C-terminal parts of the myelin basic protein. (A) Amino acid sequence of the MBP. Overlapping peptides of the epitope library are indicated. Two epitopes of the HLA-DRB1*01:01 are marked by green and blue. Brackets correspond to the sequences of peptides associated with HLA exposed on the dendritic cells isolated from normal individual with heterozygous genotype HLA-DRB1*01:01/*15:01 according to LC-MS/MS analysis. (B) Analysis of binding of HLA-DRB1*15:01 (gray bars) and HLA-DRB1*01:01 (white bars) (150 nM) with the MBP epitope library (750 nM). Trx denotes thioredoxin. Standard deviation is indicated. (C) Recognition of the chemically synthesized peptides representing MBP fragments and HA306−318 peptide (750 nM) by the HLA-DRB1*15:01 (gray bars) and HLA-DRB1*01:01 (white bars) (150 nM). Black bars represent background signal (PBS). Standard deviation is indicated. (D,E) Binding of the thioredoxin-fused peptides and its variants (750 nM) with alanine point substitutions representing MBP146−170 (D) and MBP81−104 (E) with HLA-DRB1*01:01 (150 nM). Open bars represent background signal. Standard deviation is indicated. Point mutations are indicated by different overtones.