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. 2020 Jan 17;13:1433. doi: 10.3389/fnins.2019.01433

FIGURE 2.

FIGURE 2

Chemogenetic stimulation of the LPO promotes cocaine seeking. (A) Timeline of behavioral procedures. SA, self-administration; FR, fixed ratio (number of responses required to obtain one cocaine infusion, depicted by pink lines). (B) Representative image of hM3Dq-mCitrine fluorescence in the LPO. (C) Localization of viral expression for GFP (gray) and hM3Dq (purple). (D) Cocaine self-administration behavior. Both groups acquired cocaine self-administration and there was no difference between groups across self-administration or over the last 3 days of self-administration (SA Pre). (E) Extinction behavior. Both groups extinguished responding on the previously active hole. There was no difference between groups across extinction or over the last 3 days of extinction (Ext Pre). (F) Extinction test (Ext Test). In the hM3Dq group, stimulating the LPO with CNO reinstated cocaine seeking behavior, observed as increased responding on the previously active hole (HSD, ∗∗∗P < 0.001) but not inactive hole (HSD, P = 0.99). Symbols are means ± SEM for each group; lines are individual subjects. See main text for detailed statistics.