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Filipin biosynthetic gene cluster of TR1341. The PKS I genes shown in black, tailoring genes in gray, regulatory in white. The gene annotation indicated in the table on the right and both compounds produced shown on the left. The PKSI genes disruption scheme shown in the middle A1-A5 genes replaced with the aadA spectinomycin resistance gene in TR1341ΔFIL2. Primers used to verify the gene disruption indicated by black arrows.
