FIGURE 8.

Actinomycin X2 production is substantially augmented in the TR1341ΔFIL2 mutant. LC-MS analysis of the acetone/ethylacetate extracts of wt and filipin mutant grown under standard conditions in GYM. Relative intensity of the actinomycin X2 signal in the TR1341ΔFIL2 mutant (A) is approximately 5 times higher than in the wt (C). The molecular mass of the corresponding peaks is given in (B,D). Absorption spectrum of the compound (E) corresponds to that of actinomycin X2 (F) (Furukawa et al., 1968).