Figure 7.

Conversion of tdTomato⁻ conventional CD4⁺ T cells to eTregs in vivo. 5 × 10⁶ CD4⁺CD25⁻tdTomato⁻ T cells were purified from the spleen of Tlox/Ox40Cre mice and adoptively transferred to lethal irradiated Th1.1+ hosts together with 1 × 10⁷ T cell-deleted Bone marrow cells. Three weeks later, donor cells were harvested from spleens of the hosts. The tdTomato and Foxp3 expression were analyzed in the donor cell population. (A) Sorting strategy of CD4⁺CD25⁻tdTomato⁻ donor T cells. (B) The tdTomato expression in the donor-derived CD4⁺CD25⁻ and CD4⁺CD25⁺ T cells. (C) Statistics of tdTomato expression in donor-derived CD4⁺CD25⁻ and CD4⁺CD25⁺ T cells. (D) FACS plots for foxp3 expression in the donor-derived CD25⁻ and CD25⁺ T cells. Data were collected from three independent mice.