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. 2020 Jan 10;29(2):75–89. doi: 10.1089/scd.2019.0098

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© Sherri M. Biendarra-Tiegs et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — Non-CMs do not have a notable influence on CM electrophysiology through paracrine effects. (A-E) Analysis of optical AP amplitude (A), maximum upstroke velocities (V_max) (B), APD₅₀ (AP duration at 50% repolarization) (C), APD₉₀ (D), and APD₉₀/APD₅₀ (E) for CMs cultured with conditioned media from CMs, non-CMs, co-culture with 1 part non-CM to 9 parts CM (1:9), or 7:3 co-culture. Data were collected from six independent differentiations representing five clones from two unrelated individuals between days 38 and 40 of differentiation. CM media: n = 121 cells; 1:9 media: n = 118 cells; and 7:3 media: n = 121 cells; non-CM media: n = 119 cells. Heavier dashed lines within each violin indicate medians and lighter dashed lines indicate interquartile range. Data were initially analyzed by a Kruskal-Wallis test (P < 0.01 for V_max, P < 0.001 for APD₉₀/APD₅₀, and n.s. for all others) and pairwise P values were calculated by Mann-Whitney U tests for V_max and APD₉₀/APD₅₀ (*P ≤ 0.0083; **P ≤ 0.001).