Figure 2.

FAMIN Has Adenosine Deaminase, Purine Nucleoside Phosphorylase, and S-Methyl-5′-Thioadenosine (MTA) Phosphorylase Activities

(A) Representative extracted chromatograms, using normalized peak intensity, for adenosine (top chromatogram); and inosine, hypoxanthine, and R1P (bottom chromatogram) following incubation of FAMIN^254I or control with 100 μM adenosine.

(B) Adenosine, inosine, hypoxanthine, and R1P levels following incubation of recombinant FAMIN^254I or control as per (A) (n = 3).

(C) Representative extracted chromatograms for adenine using a modified CSH-C18 method.

(D) Fractional conversion of adenosine into its products following incubation of Strep-tagged FAMIN^254I with 100 μM adenosine (n = 3, mean).

(E) Adenosine levels in reactions of adenine and R1P in the presence of Strep-tagged FAMIN^254I or control (n = 3).

(F) FAMIN-catalyzed enzymatic reactions.

(G) FAMIN activity toward purine and pyrimidine nucleosides, measured as substrate (each added at 100 μM) consumption (SAM; S-adenosylmethionine; 2′-dA, 2′-deoxyadenosine; n = 3).

(H) Representative extracted chromatograms for inosine, hypoxanthine, and R1P (top chromatogram); and MTA, adenine, and methylthioribose-1-phosphate (bottom chromatogram) upon incubation of inosine and MTA, respectively, with recombinant FAMIN^254I.

(I) Adenine and methylthioribose-1-phosphate levels upon incubation of MTA with FAMIN^254I or buffer control (n = 3).

(J) Further FAMIN-catalyzed enzymatic reaction.

Data represented as mean ± SEM. ^∗p < 0.05 (unpaired, two-tailed Student’s t test).