Figure S6.

A Purine Nucleotide Cycle Operates in Macrophages and HepG2 Cells, Related to Figure 5

(A) Heatmap of metabolites in control and FAMIN-silenced HepG2 cells 48 h after transfection (n = 6).

(B) Ampd2, Ampd3, Adss, Adsl and Aprt expression in Famin^p.254I, Famin^p.254V, Famin^p.284R M0, M1 and M2 macrophages (n = 5). Ampd: AMP deaminase; Adss: Adenylosuccinate synthase; Adsl: Adenylosuccinate lyase; Aprt: Adenine phosphoribosyltransferase.

(C) Extracellular acidification rate (ECAR) of Famin^p.254I M0 macrophages silenced for Adss, Adsl and Ampds (Ampd1, 2 and 3) or transfected with a non-targeting scrambled siRNA control. Basal ECAR measurement was followed by sequential treatment (dotted vertical lines) with oligomycin A (Oligo) (n = 3). Ampd: AMP deaminase; Adss: Adenylosuccinate synthase; Adsl: Adenylosuccinate lyase.

(D) Oxygen consumption rate (OCR), and extracellular acidification rate (ECAR) with maximal respiratory and glycolytic capacities of control and FAMIN-silenced HepG2 cells treated with 10 μM, 20 μM, 60 μM, 100 μM of L-alanosine or vehicle control for 24 h. Basal OCR and ECAR measurements followed by sequential treatment (dotted vertical lines) with oligomycin A (Oligo), FCCP, and rotenone plus antimycin A (Rot + ant) (n = 3).

Data are represented as mean ± SEM.