Figure S5.

Latrophilin1 Interaction with Teneurins and FLRTs in Trans Induces Repulsion, Related to Figure 5

(A) E15.5 dissociated cortical neurons were grown on alternate stripes containing FC (black stripes) and wild-type or mutant TL or FL murine Lphn1 Lec-Olf protein (red stripes). Neurons were stained with anti-beta-III-tubulin to visualize neurites (green) and DAPI (white). High magnification showing the location of nuclei (DAPI, white) on stripes is shown at the bottom. Red stripes, which contain Lphn protein, are indicated with yellow arrowheads. After imaging, the percentage of DAPI+ pixels on red stripes was quantified and it is shown in Figure 5B. (B) E15.5 cortical explants were grown on the same stripes as in (A) and the quantification of the results is shown Figure 5F. White arrow heads indicate the red stripes which contain Lphn1 mutant protein. Scale bars represent 20 μm (A) and 200 μm (B).