Figure 3.

Gch1 overexpression enhances folate pathway derivatives but does not affect known ferroptosis regulators. (A) Protein levels by heat map of known ferroptosis regulators upon 0.3 μM RSL3 treatment for 3 h in Gch1-overexpressing MF cells (Gch1 OE) compared to control cells (control). Data were average of n = 4 or 5 independent biological replicates per condition. (B) Partial least-squares discriminant analysis of Gch1 OE cells compared to control and volcano plot of whole-cell-shotgun metabolomics analysis. (C) Upregulated metabolic precursors of BH₄ and BH₂ in KEGG pathway of GCH1 in Gch1 OE cells compared to control (n = 5 independent biological replicates).