Table 3.
Forward and reverse primer sequences used for quantitative reverse transcription PCR
| Gene1 | Direction2 | Primer sequence | Reference |
|---|---|---|---|
| Internal control gene | |||
| β-ACTIN | F | 5′-CAC GCC ATC CTG CGT CTG GA-3′ | Fry et al. (2012) |
| R | 5′-AGC ACC GTG TTG GCG TAG AG-3′ | ||
| GAPDH | F | 5′-CAG CAA TGC CTC CTG TAC CA-3′ | Vigors et al. (2014) |
| R | 5′-ACG ATG CCG AAG TTG TCA TG-3′ | ||
| Target gene | |||
| FAS | F | 5′-CAC AAC TCC AAA GAC ACG-3′ | Kellner et al. (2017) |
| R | 5′-AGG AAC TCG GAC ATA GCG-3′ | ||
| CD36 | F | 5′-CTG GTG CTG TCA TTG GAG CAG T-3′ | Li et al. (2017) |
| R | 5′-CTG TCT GTA AAC TTC CGT GCC TGT T-3′ | ||
| ACC | F | 5′-ATG GAT GAA CCG TCT CCC-3′ | Kellner et al. (2017) |
| R | 5′-TGT AAG GCC AAG CCA TCC-3′ | ||
| PPAR-α | F | 5′-GCC GAA GTC ATC CAA GAA GG-3′ | Kellner et al. (2017) |
| R | 5′-TGA CCT CAC AGG ACA CTC CAA G-3′ | ||
| CPT1A | F | 5′-GCA TTT GTC CCA TCT TTC GT-3′ | Varady et al. (2012) |
| R | 5′-GCA CTG GTC CTT CTG GGA TA -3′ | ||
| FABP1 | F | 5′-ACA TCA AGG GGA CAT CGG-3′ | Kellner et al. (2017) |
| R | 5′-GTC TCC ATC TCA CAC TCC-3′ | ||
| CPT1B | F | 5′-GGA CGA GGA GTC TCA CCA CTA TGA C-3′ | Li et al. (2017) |
| R | 5′-TCT TGA ACG CGA TGA GGG TGA-3′ | ||
| FATP1 | F | 5′-CCC TCT GCG TCG CTT TGA TG-3′ | Li et al. (2017) |
| R | 5′-GCT GCG GTC CCG GAA ATA CA-3′ | ||
| FABP3 | F | 5′-CCA ACA TGA CCA AGC CTA CCA CA-3′ | Li et al. (2017) |
| R | 5′-ACA AGT TTG CCT CCA TCC AGT G-3′ | ||
| FABP4 | F | 5′-CAG GAA AGT CAA GAG CAC CA-3′ | Zhao et al. (2010) |
| R | 5′-TCG GGA CAA TAC ATC CAA CA-3′ | ||
| PPAR-γ | F | 5′-GTG GAG ACC GCC CAG GTT TG-3′ | Li et al. (2017) |
| R | 5′-GGG AGG ACT CTG GGT GGT TCA-3′ | ||
| LPL | F | 5′-CCC TAT ACA AGA GGG AAC CGG AT-3′ | Li et al. (2017) |
| R | 5′-CCG CCA TCC AGT CGA TAA ACG T-3′ | ||
| HSL | F | 5′-AAC GCA ATG AAA CAG GCC-3′ | Kellner et al. (2017) |
| R | 5′-TGT ATG ATC CGC TCA ACT CG-3′ |
1 GAPDH, glyceraldehyde 3-phosphate dehydrogenase; FAS, fatty acid synthase; CD36, cluster of differentiation 36/fatty acid translocase; ACC, acetyl CoA carboxylase; PPAR-α, peroxisome proliferator-activated receptor alpha; CPT1A, carnitine palmitoyl transferase 1 A; FABP1, fatty acid binding protein 1; CPT1B, carnitine palmitoyl transferase 1 B; FATP1, fatty acid transport protein 1; FABP3, fatty acid binding protein 3; FABP4, fatty acid binding protein 4; PPAR-ɤ, peroxisome proliferator-activated receptor gamma; LPL, lipoprotein lipase; HSL, hormone sensitive lipase.
2Direction of primer (F, forward; R, reverse).