Figure 2.

CiTLR22a and CiTLR22b bind dsRNA analog poly(I:C). Cell lysates of overexpressed CiTLR22a and CiTLR22b were incubated with biotin-poly(I:C) (100 ng/ml) or unlabeled poly(I:C) at pH 5.0 (A) or pH 7.4 (B) for 1 h. Complexes were pulled down by streptavidin beads and analyzed by Western blotting (WB) with anti-myc Ab. (C,D) The binding abilities between CiTLR22a-LRR, CiTLR22b-LRR, and different pathogen-associated molecular patterns (PAMPs) were analyzed by ELISA at pH 5.0. Data shown are representative of at least three independent experiments.