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CiTLR22a and CiTLR22b regulate the protein and phosphorylation levels of IRF3 and IRF7 by WB. CIK cells were transfected with pCiTLR22a-myc or pCiTLR22b-myc in 6-well plates for 24 h incubation, then stimulated with poly(I:C) for 24 h. WB was conducted with anti-IRF7 (A) and anti-IRF3 antiserums (B), respectively. “-P” indicates the phosphorylation. All the experiments were repeated triplicately.
