Figure 10.

Evaluation of proteins involved in iron metabolism and iron content in conditional KO mouse sciatic nerves. Representative Western blots for DMT1, Fth, and Tfr1 made with total proteins purified from the sciatic nerves of controls, DMT1^KO (A), Fth^KO (B), and Tfr1^KO (C) at P15. D, Representative Western blots for DMT1, Fth, and Tfr1 made with total proteins purified from controls, DMT1^KO, Fth^KO, and Tfr1^KO sciatic nerves at P90. p84 was used as the internal standard, and data from 4 independent experiments are summarized based on the relative spot intensities and plotted as percentage of controls. E, Total iron content was examined in sciatic nerve homogenates from controls, DMT1^KO, Fth^KO, and Tfr1^KO at P15 using a colorimetric iron assay. F, Perl's staining in representative teased sciatic nerve fibers collected from controls, DMT1^KO, Fth^KO, and Tfr1^KO at P30. G, The average Perl's intensity staining per fiber was quantified in all genotypes at P15 and P30. Data are mean ± SEM of at least 4 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; versus control.