Fig. 5.

N-acetylcysteine decreases neutrophil transmigration across the choroidal epithelial barrier. Choroid plexus epithelial cells were pretreated with saline, P3C or P3C + NAC for 14 h. Total white blood cells were isolated from P8 rats treated with P3C for 14 h and added to the stroma-mimicking basolateral medium in the upper chamber. Migrated cells were recovered from the CSF-mimicking apical medium in the lower chamber after five hours, PMN were labeled and counted. Representative data are shown as mean ± SEM (n = 4). ** p < 0.01; ***p < 0.001, one-way ANOVA followed by Tukey’s multiple comparisons test. The experiment was repeated with different preparations of choroidal epithelial and white blood cells, and gave similar statistically significant differences